Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation

Mitsuko Hayashi-Nishino, Naonobu Fujita, Takeshi Noda, Akihito Yamaguchi, Tamotsu Yoshimori, Akitsugu Yamamoto

研究成果: Short survey査読

62 被引用数 (Scopus)

抄録

The origin and source of autophagosomal membranes are long-standing questions. By electron microscopy, we show that the endoplasmic reticulum (ER) associates with early autophagic structures called isolation membranes (IM) or phagophores in mammalian culture cells. Overexpression of a mutant of Atg4B, which causes defects in autophagosome formation, caused accumulation of ER-IM complexes. Electron tomography revealed the ER-IM complex as a subdomain of the ER forming a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.

本文言語English
ページ(範囲)301-303
ページ数3
ジャーナルAutophagy
6
2
DOI
出版ステータスPublished - 2010 2月 16
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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