Electron spin resonance study of the binding structures of cationic water-soluble metalloporphyrins on highly oriented deoxyribonucleic acid fibres

Makoto Chikira, Shoichi Suda, Toyotaka Nakabayashi, Yoshihito Fujiwara, Takashi Ejiri, Mitsuhide Yoshikawa, Nagao Kobayashi, Heisaburo Shindo

研究成果: Article査読

22 被引用数 (Scopus)

抄録

The binding structures of several paramagnetic metal complexes of 5,10,15,20-tetrakis(1-methyl-4-or -2-pyridinio) porphyrin (H2L 1 or H2L2) on the highly oriented DNA fibres were investigated by means of ESR spectroscopy and computer simulations of the spectra. The structural parameters characteristic of the sterospecific groove binding were obtained for [CuL2]Cl4, high-spin [FeCl(L1)]Cl4 and low-spin [CoL1] [ClO 4]4 and the low-spin monoimidazole adduct [CoL 1(Him)] [ClO4]4. They bind to the DNA with the normals of the porphyrin planes titled by about 45° from the double helix axes. The low-spin bis(imidazole) adduct [FeCl(L1) (Him) 2]Cl4 binds to A-form DNA with the porphyrin plane nearly parallel to the double helix axis. The complex [CoL1] [ClO 4]4 binds to DNA as an intercalator as well as a groove binder. It was also revealed that some metalloporphyrins stabilize the tertiary structures of DNA around the binding sites.

本文言語English
ページ(範囲)1325-1331
ページ数7
ジャーナルJournal of the Chemical Society, Dalton Transactions
8
DOI
出版ステータスPublished - 1995 12月 1

ASJC Scopus subject areas

  • 化学 (全般)

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