Electrochemicolor imaging of endogenous alkaline phosphatase and respiratory activities of mesenchymal stem cell aggregates in early-stage osteodifferentiation

Kosuke Ino, Takehiro Onodera, Yusuke Kanno, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue, Hitoshi Shiku

研究成果: Article

14 引用 (Scopus)

抜粋

Mesenchymal stem cells (MSCs) have received great attention for tissue engineering. In the present study, the early stage of osteogenesis of MSC aggregates was monitored by electrochemicolor imaging using an electrode array device that can simultaneously detect multiple redox species. Electrochemicolor imaging was used to evaluate the cells’ endogenous alkaline phosphatase (ALP) and respiratory activities. High ALP activity was observed in the aggregates after osteomedium (OM) treatment, with hydroxyapatite addition inducing early-stage osteogenesis in large aggregates. In small aggregates, OM treatment decreased the respiratory activity. To the best of our knowledge, this is the first report to use electrochemical imaging of ALP and respiratory activities to evaluate osteogenesis in MSC aggregates.

元の言語English
ページ(範囲)554-561
ページ数8
ジャーナルElectrochimica Acta
268
DOI
出版物ステータスPublished - 2018 4 1

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

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