TY - JOUR
T1 - Electrochemical/photochemical formation of enzyme patterns on glass substrates using a scanning electrochemical/confocal microscope
AU - Oyamatsu, Daisuke
AU - Kanaya, Norihiro
AU - Shiku, Hiroshi
AU - Nishizawa, Matsuhiko
AU - Matsue, Tomokazu
PY - 2003/6/1
Y1 - 2003/6/1
N2 - A hybrid system consisting of a scanning electrochemical microscope (SECM) and scanning confocal microscope (SCFM) was fabricated and used for micropatterning and imaging of diaphorase immobilized on a glass substrate. Simultaneous imaging of the diaphorase spots demonstrated that the SECM can provide information on a localized enzyme reaction, while the SCFM affords information on the location of the active enzyme. By using this SECM/SCFM system, spatially selective deactivation of diaphorase was performed by inducing a local electrochemical reaction or by irradiating with the focused laser. The resulting patterns of diaphorase were simultaneously imaged with the SECM/SCFM system.
AB - A hybrid system consisting of a scanning electrochemical microscope (SECM) and scanning confocal microscope (SCFM) was fabricated and used for micropatterning and imaging of diaphorase immobilized on a glass substrate. Simultaneous imaging of the diaphorase spots demonstrated that the SECM can provide information on a localized enzyme reaction, while the SCFM affords information on the location of the active enzyme. By using this SECM/SCFM system, spatially selective deactivation of diaphorase was performed by inducing a local electrochemical reaction or by irradiating with the focused laser. The resulting patterns of diaphorase were simultaneously imaged with the SECM/SCFM system.
KW - Diaphorase
KW - Patterning
KW - SCFM
KW - SECM
UR - http://www.scopus.com/inward/record.url?scp=12444344282&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12444344282&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(03)00089-3
DO - 10.1016/S0925-4005(03)00089-3
M3 - Article
AN - SCOPUS:12444344282
VL - 91
SP - 199
EP - 204
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1-3
ER -