TY - JOUR
T1 - Electrochemical studies of danthron and the DNA-danthron interaction
AU - Zhou, Hui
AU - Sun, Ziyi
AU - Hoshi, Tomonori
AU - Kashiwagi, Yoshitomo
AU - Anzai, Junichi
AU - Li, Genxi
N1 - Funding Information:
We greatly appreciate the support of the National Natural Science Foundation, and the Ministry of Education P.R. China for this research.
PY - 2005/4/1
Y1 - 2005/4/1
N2 - Danthron is an important natural occurring component in laxative drugs. In this paper, electrochemical investigation of danthron and its interaction with DNA is reported. Via the electrochemical approach assisted by ultraviolet-visible (UV-Vis) spectroscopy, we have proved that danthron intercalates into DNA strands forming some nonelectroactive complexes, which results in the decrease of redox peak currents of danthron. In addition, the decrease of the peak currents is proportional to the concentration of DNA. The difference between the interaction of danthron with double-stranded DNA (dsDNA) and with single-stranded DNA (ssDNA) has also been studied. This character implies the potential of danthron to discriminate dsDNA and ssDNA.
AB - Danthron is an important natural occurring component in laxative drugs. In this paper, electrochemical investigation of danthron and its interaction with DNA is reported. Via the electrochemical approach assisted by ultraviolet-visible (UV-Vis) spectroscopy, we have proved that danthron intercalates into DNA strands forming some nonelectroactive complexes, which results in the decrease of redox peak currents of danthron. In addition, the decrease of the peak currents is proportional to the concentration of DNA. The difference between the interaction of danthron with double-stranded DNA (dsDNA) and with single-stranded DNA (ssDNA) has also been studied. This character implies the potential of danthron to discriminate dsDNA and ssDNA.
KW - DNA
KW - Danthron
KW - Electrochemistry
KW - Intercalation
KW - UV-Vis spectroscopy
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U2 - 10.1016/j.bpc.2004.11.001
DO - 10.1016/j.bpc.2004.11.001
M3 - Article
C2 - 15792857
AN - SCOPUS:15544385196
VL - 114
SP - 21
EP - 26
JO - Biophysical Chemistry
JF - Biophysical Chemistry
SN - 0301-4622
IS - 1
ER -