Electrochemical imaging using redox mediators for cell activity of three-dimensional cultured cells

Kosuke Ino, Takehiro Onodera, Mika T. Fukuda, Yuji Nashimoto, Hitoshi Shiku

研究成果: Conference contribution

抄録

Three-dimensional (3D) cultured cells are widely used for tissue engineering and drug screening. Therefore, a method for evaluating them is desired. Several kinds of tools have been developed. Among them, electrochemical approaches are receiving considerable attention because of their great features, such as simplicity and highly sensitive detection. Especially, electrode array devices have been developed for electrochemical imaging of cell activity. In he electrochemical imaging, cell activity is converted to current values at electrochemical sensors, and we obtain 2D images consisting of the currents, which is called as electrochemical images. The electrochemical images are useful for chemical mapping. We previously reported a CMOS-based amperometric device containing 400 sensors for electrochemical imaging. The device was applied for electrochemical imaging of several kinds of cell activity. In this study, the device was applied for evaluating intracellular activity using redox mediators. To access intracellular compounds, hydrophobic mediators were used because the mediators can penetrate cell membranes. First, ferrocenemethanol (FMA) was used as a hydrophobic mediator and oxidation currents of FMA were measured. However, the detection was unsuitable for evaluating intracellular enzymes and/or redox compounds. Next, a double-mediator system of [Fe(CN)6]3- and menadione was adapted for measuring intracellular sensing. The detection is deeply discussed in the oral presentation. Briefly, quinone oxidoreductase 1 (NQO1) activity was successfully detected. In the future, the detection system will be used for drug screening.

本文言語English
ホスト出版物のタイトルAdvanced Environmental, Chemical, and Biological Sensing Technologies XV
編集者Tuan Vo-Dinh
出版社SPIE
ISBN(電子版)9781510626799
DOI
出版ステータスPublished - 2019
イベントAdvanced Environmental, Chemical, and Biological Sensing Technologies XV 2019 - Baltimore, United States
継続期間: 2019 4 142019 4 16

出版物シリーズ

名前Proceedings of SPIE - The International Society for Optical Engineering
11007
ISSN(印刷版)0277-786X
ISSN(電子版)1996-756X

Conference

ConferenceAdvanced Environmental, Chemical, and Biological Sensing Technologies XV 2019
国/地域United States
CityBaltimore
Period19/4/1419/4/16

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • コンピュータ サイエンスの応用
  • 応用数学
  • 電子工学および電気工学

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