TY - JOUR
T1 - Electric field effects on fluorescence of the green fluorescent protein
AU - Nakabayashi, Takakazu
AU - Kinjo, Masataka
AU - Ohta, Nobuhiro
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research in Priority Area ‘Molecular Nano Dynamics’ from the Ministry of Education, Culture, Sports, Science, and Technology in Japan.
PY - 2008/5/27
Y1 - 2008/5/27
N2 - External electric field effects on state energy and photoexcitation dynamics have been examined for a mutant of UV-excited green fluorescent protein (GFPuv5) in a PVA film. The electrofluorescence spectrum of GFPuv5 is reproduced by a linear combination between the fluorescence spectrum and its second derivative spectrum, indicating the field-induced fluorescence quenching and the difference in electric dipole moment between the fluorescent state and the ground state. The direct measurements of the field-induced change in fluorescence decay show that the field-induced quenching results from the field-induced increase in the rate of the non-radiative process from the fluorescent state.
AB - External electric field effects on state energy and photoexcitation dynamics have been examined for a mutant of UV-excited green fluorescent protein (GFPuv5) in a PVA film. The electrofluorescence spectrum of GFPuv5 is reproduced by a linear combination between the fluorescence spectrum and its second derivative spectrum, indicating the field-induced fluorescence quenching and the difference in electric dipole moment between the fluorescent state and the ground state. The direct measurements of the field-induced change in fluorescence decay show that the field-induced quenching results from the field-induced increase in the rate of the non-radiative process from the fluorescent state.
UR - http://www.scopus.com/inward/record.url?scp=43549122786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43549122786&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2008.04.018
DO - 10.1016/j.cplett.2008.04.018
M3 - Article
AN - SCOPUS:43549122786
VL - 457
SP - 408
EP - 412
JO - Chemical Physics Letters
JF - Chemical Physics Letters
SN - 0009-2614
IS - 4-6
ER -