Electric field effects on fluorescence of the green fluorescent protein

Takakazu Nakabayashi, Masataka Kinjo, Nobuhiro Ohta

研究成果: Article査読

17 被引用数 (Scopus)

抄録

External electric field effects on state energy and photoexcitation dynamics have been examined for a mutant of UV-excited green fluorescent protein (GFPuv5) in a PVA film. The electrofluorescence spectrum of GFPuv5 is reproduced by a linear combination between the fluorescence spectrum and its second derivative spectrum, indicating the field-induced fluorescence quenching and the difference in electric dipole moment between the fluorescent state and the ground state. The direct measurements of the field-induced change in fluorescence decay show that the field-induced quenching results from the field-induced increase in the rate of the non-radiative process from the fluorescent state.

本文言語English
ページ(範囲)408-412
ページ数5
ジャーナルChemical Physics Letters
457
4-6
DOI
出版ステータスPublished - 2008 5 27
外部発表はい

ASJC Scopus subject areas

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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