Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases

Satoshi Ansai, Tetsushi Sakuma, Takashi Yamamoto, Hiroyoshi Ariga, Norihito Uemura, Ryosuke Takahashi, Masato Kinoshita

研究成果: Article査読

90 被引用数 (Scopus)

抄録

Transcription activator-like effector nucleases (TALENs) have become powerful tools for targeted genome editing. Here we demonstrate efficient targeted mutagenesis in medaka (Oryzias latipes), which serves as an excellent vertebrate model for genetics and genomics. We designed and constructed a pair of TALENs targeting the medaka DJ-1 gene, a homolog of human DJ-1 (PARK7). These TALENs induced a number of insertions and deletions in the injected embryos with extremely high efficiency. This induction of mutations occurred in a dose-dependent manner. All screened G0 fish injected with the TALENs transmitted the TALEN-induced mutations to the next generation with high efficiency (44-100%). We also confirmed that these TALENs induced site-specific mutations because none of the mutations were found at potential off-target sites. In addition, the DJ-1 protein was lost in DJ-1Δ7/Δ7 fish that carried a TALEN-induced frameshift mutation in both alleles. We also investigated the effect of the N- and C-terminal regions of the transcription activator-like (TAL) effector domain on the gene-disrupting activity of DJ1-TALENs and found that 287 amino acids at the N terminus and 63 amino acids at the C terminus of the TAL domain exhibited the highest disrupting activity in the injected embryos. Our results suggest that TALENs enable us to rapidly and efficiently establish knockout medaka strains. This is the first report of targeted mutagenesis in medaka using TALENs. The TALEN technology will expand the potential of medaka as a model system for genetics and genomics.

本文言語English
ページ(範囲)739-749
ページ数11
ジャーナルGenetics
193
3
DOI
出版ステータスPublished - 2013
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル