Efficient gene transfer by pullulan-spermine occurs through both clathrin- and raft/caveolae-dependent mechanisms

Isao Kanatani, Tomonori Ikai, Arimichi Okazaki, Jun ichiro Jo, Masaya Yamamoto, Masaaki Imamura, Akihiro Kanematsu, Shingo Yamamoto, Noriyuki Ito, Osamu Ogawa, Yasuhiko Tabata

研究成果: Article査読

72 被引用数 (Scopus)

抄録

The feasibility and mechanism of gene delivery by pullulan-spermine, a recently developed cationic polysaccharide, were investigated. Pullulan-spermine-mediated transfection of plasmid DNA resulted in greatly reduced cytotoxicity and a 10-fold increase in the level of gene expression when compared to Lipofectamine 2000, a commercially available cationic lipid. Additionally, after transfection of p53-expressing plasmid DNA by pullulan-spermine but not Lipofectamine 2000, the in vitro proliferation of T24 cells was significantly reduced. Pullulan-spermine-mediated gene expression was inhibited by both chlorpromazine of clathrin-mediated endocytosis inhibitor and methyl-β-cyclodextrin and filipin of raft/caveolae inhibitors. We conclude that pullulan-spermine is a promising carrier for gene transfection, and that cellular uptake of pullulan-spermine-plasmid DNA complexes is mediated by clathrin- and raft/caveolae-dependent endocytotic pathways.

本文言語English
ページ(範囲)75-82
ページ数8
ジャーナルJournal of Controlled Release
116
1
DOI
出版ステータスPublished - 2006 11月 10
外部発表はい

ASJC Scopus subject areas

  • 薬科学

フィンガープリント

「Efficient gene transfer by pullulan-spermine occurs through both clathrin- and raft/caveolae-dependent mechanisms」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル