Effects of the Protein Phosphatase Inhibitors Okadaic Acid and Calyculin A on Insulin Release from Rat Pancreatic Islets

Tatsuo Tamagawa, Akihisa Iguchi, Kazumasa Uemura, Hisayuki Miura, Katsunori Nonogaki, Toshiaki Ishiguro, Nobuo Sakamoto

研究成果: Article査読

15 被引用数 (Scopus)

抄録

The role of protein phosphatases in the regulation of insulin release from rat pancreatic islets was studied with protein phosphatase inhibitors, okadaic acid and calyculin A. Okadaic acid inhibited glucose- and glyceraldehyde-induced insulin release dose-dependently and also inhibited the potentiation of glucose-induced release either by adding forskolin, an activator of adenylate cyclase or by increasing K+ concentration to 25 mM. At a non-stimulatory concentration of 3 mM glucose, a high concentration (2 μM) of okadaic acid inhibited insulin release induced by high K+ or 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, but a low concentration (1 μM) of okadaic acid did not significantly inhibit TPA-induced insulin release. Calyculin A also inhibited glucose-induced insulin release, and the effect was greater than that of okadaic acid. The data suggest that protein phosphatases may play an important role in the regulation of insulin releae.

本文言語English
ページ(範囲)325-329
ページ数5
ジャーナルEndocrinologia Japonica
39
3
DOI
出版ステータスPublished - 1992
外部発表はい

ASJC Scopus subject areas

  • 内分泌学
  • 工学(全般)

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