The effects of the change of flow direction on the morphology of cultured bovine aortic endothelial cells were studied. Fully confluent endothelial cells cultured on glass plates were subjected to fluid-imposed shear stress of 2 Pa for 24 hours. Experiments on shear flow exposure were performed for alternating orthogonal flow with a 3, 30 min and 1 hour interval. (Data of 30 min interval were previously reported.) After flow exposure, endothelial cells were fixed and F-actin filaments were stained with rhodamine phalloidin and shape index (SI) and angle of cell orientation were measured. After application of 2 Pa shear stress for 24 hours in the alternating orthogonal flow with 30 min interval the endothelial cells elongated (SI=0.63±0.14) and slightly aligned with the center of two flow directions. With 1 hour interval, cells were more elongated (0.55±0.16) than the cells with 30 min and aligned with the center of two flow directions. In the both flow conditions, thick and clear stress fibers which aligned with cell axes were observed in central portion of the cells. In the flow experiments with 3 min interval, many cells detached from the glass plates. Moreover, in the remained cells on the glass plates after 24 hours, stress fiber was not observed. The results of this study indicate that morphology of endothelial cells are affected with the change of flow direction. In the atherosclerotic regions, such as arterial bends and branches, the blood flow is not simple but very complex with reverse and secondary flows. From these points of view, the change of the flow direction may affect the morphology and function of endothelial cells in in vivo flow condition.
|ジャーナル||japanese journal of medical electronics and biological engineering|
|出版ステータス||Published - 1998 9 1|
ASJC Scopus subject areas
- Biomedical Engineering