Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer

Keitaro Tanoi, Natsuko I. Kobayashi, Takayuki Saito, Naoko Iwata, Risa Kamada, Ren Iwata, Hisashi Suzuki, Atsushi Hirose, Yoshimi Ohmae, Ryohei Sugita, Tomoko M. Nakanishi

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Aims: The mechanisms underlying magnesium (Mg) uptake by plant roots remain to be fully elucidated. In particular, there is little information about the effects of Mg deficiency on Mg uptake activity. A Mg uptake kinetic study is essential for better understanding the Mg uptake system.

Methods: We performed a Mg uptake tracer experiment in rice plants using 28 Mg.

Results: Mg uptake was mediated by high- and low-affinity transport systems. The Km value of the high-affinity transport system was approximately 70 μM under Mg-deficient conditions. The Mg uptake activity was promoted by Mg deficiency, which in turn fell to the basal level after 5- min of Mg resupply. The induced uptake rate was inhibited by ionophore treatment, suggesting that an energy-dependent uptake system is enhanced by Mg deficiency.

Conclusions: The Mg uptake changes rapidly with Mg conditions in rice, as revealed by a 28 Mg tracer experiment. This technique is expected to be applicable for Mg uptake analyses, particularly in mutants or other lines.

本文言語English
ページ(範囲)69-77
ページ数9
ジャーナルPlant and Soil
384
1-2
DOI
出版ステータスPublished - 2014 10月 25

ASJC Scopus subject areas

  • 土壌科学
  • 植物科学

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