Effects of L-NAME and L-arginine on diaphragm contraction in a septic animal model

C. Shindoh, D. Wu, Y. Ohuchi, H. Kurosawa, Y. Kikuchi, W. Hida, K. Shirato

研究成果: Article査読

8 被引用数 (Scopus)


The effects of nitric oxide on diaphragm contraction after endotoxin administration were studied in Wistar rats. The animals were divided into seven treatments: a saline-injected group as control, three groups injected with L-NAME (0.01, 0.1, 1 mg/kg) and three groups injected with L-arginine (1, 10, 100 mg/kg). Escherichia coli endotoxin was injected into the peritoneal cavity 15 min later. Twitch kinetics and force-frequency curves were measured 0, 2, and 4 hr after endotoxin injection. In the control group, the force-frequency curves significantly decreased from 0 hr to 4 hr. In the L-NAME group, the force-frequency curves at 4 hr showed significant increases in a dose-dependent manner. In the L-arginine group, the force-frequency curve with 100 mg/kg at 4 hr showed a significant increase. There was no consistent change in the contraction time, half relaxation time, or fatiguability. NADPH diaphorase histochemistry performed on diaphragm muscle samples 4 hr after endotoxin injection showed positive in the control and L- arginine group, but was only weakly observed in L-NAME group. These data suggest that nitric oxide contributes to the endotoxin induced diaphragm contractile deterioration.

ジャーナルComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
出版ステータスPublished - 1998 1

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 分子生物学


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