Dispersed rat pancreatic acini were used to determine the effect of galanin on the exocrine pancreas and on basal and secretagogue-stimulated amylase secre-tion. Basal amylase secretion and amylase release stimu-lated by cholecystokinin octapeptide, bombesin, 12-o-tetradecanoyl-phorbol-13-acetate (TPA), secretin, and vasoactive intestinal peptide were not affected by galanin in doses ranging from 10-12to 10-6M. Galanin, however, significantly inhibited the amylase release stimulated by sub-and supramaximal doses of carbachol. Atime course study showed that the inhibition by galanin occurred during the sustained phase of carbachol-stimulated amylase secretion. The inhibitory action of galanin disappeared in acini obtained from animals pre-treated with pertussis toxin (PTX). These results suggest that galanin inhibits carbachol-stimulated amylase secre-tion through a mechanism related to a PTX-sensitive G protein.
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