Effects of freeze-drying of samples on metabolite levels in metabolome analyses

Akira Oikawa, Takao Otsuka, Yusuke Jikumaru, Shinjiro Yamaguchi, Fumio Matsuda, Ryo Nakabayashi, Tadashi Takashina, Kanji Isuzugawa, Kazuki Saito, Katsuhiro Shiratake

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Freeze-drying (FD) is a useful technique for removing water from biological tissues, such as food samples. Cellular components freeze at once, and the ice sublimates under conditions of high vacuum and low temperatures. Because biological activity is restricted during FD, the degradation of cellular metabolites is often believed to be limited. However, the cellular structure is damaged by several factors, such as the increase in cell volume during freezing, and this has serious effects on the levels of some cellular metabolites. We studied these effects of FD on metabolite levels when using it as a sample preparation step in metabolome analysis. We observed significant decreases in the levels of some metabolites, such as succinate and choline, in Arabidopsis and pear, respectively. We also found that the effects of FD on certain metabolite levels differed between Arabidopsis plants and pear fruits. These results suggest that it is necessary to confirm the metabolite recovery in each sample species when FD is used for sample preparation.

本文言語English
ページ(範囲)3561-3567
ページ数7
ジャーナルJournal of Separation Science
34
24
DOI
出版ステータスPublished - 2011 12月 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • ろ過および分離

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