We examined the effects of short-, medium- or long-chain fatty acids on the accumulation of cytosolic triacylglycerol (TAG) in bovine mammary epithelial cells (bMEC). Octanoate and long-chain fatty acids stimulated TAG accumulation in a concentration-dependent manner from 1 to 10 mM and from 50 to 400 μM, respectively, as well as mRNA expression of CD36 and UCP2. Octanoate, oleate and linoleate increased lipid droplet formation. Leptin mRNA expression was significantly reduced by addition of acetate and butyrate but was elevated by addition of oleate and linoleate. Long-chain fatty acids stimulated αs1-casein mRNA expression.
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