Effects of exercise training and enalapril on nephritic damage rats with adriamycin

Li Na Ji, Li Cao, Da Kun Chen, Kazunori Yoshida, Masahiro Kohzuki, Tokutaro Sato

研究成果: Article査読

抄録

Aim: To study the effects of exercise training and angiotensin-converting-enzyme inhibitor (ACEI), enalaprol on the progression of renal damage in rats with adriamycin nephrosis. Methods: (1) The experiment was conducted at the Department of Internal Medicine and Rehabilitation Science, Tohoku University Graduate School of Medicine between January and April 2001. Thirty-three healthy Wistar male rats aged 8 weeks were selected, and they were treated with 2 mg/kg adriamycin with injection of vein, repeated once after 20 days to make nephrosis models. From the 13rd weeks, the nephrosis rats were assigned randomly into 4 groups: control group (n=8), exercise training group (n=8), enalaprol group (n= 9) and exercise training + enalaprol group (n=8). (2) The treadmill training with 0° obliquity was performed, at the speed of 20 m per minute and 60 minutes per day, 5 days in a week for 8 weeks. Those in the enalaprol group were treated with 2 mg/kg per day enalaprol continuously in abdominal cavity. Those in the control group were neither doing exercise training nor treated with enalaprol. (3) All the rats were raised with metabolism cage. At every 2 weeks before and after exercise the body mass, systolic pressure, 24 hours drinking volume and urine volume, urine natrium, kaiu and excretion volume of urine protein were detected at the same time. At the 8th week, when the exercise was done, they were killed; The urine natrium, kaiu, excretion volume of protein and blood biochemical index were detected with auto-analyser. The kidney was gained, and after processing nephritic pathology analysis was performed under light microscope. Glomerulosclerosis index=(1 × N1 + 2 × N2 + 3 × N3 + 4 × N4)/(N0 + N1 + N2 +N3 + N4), in which the N represented number of sclerotic renal corpuscle in different degree. The calculation formula of index of renal corpuscle lipid deposition was the same to glomerulosclerosis index. (4) The difference of measurement data was compared with single factor analysis of variance; The difference of measurement data among groups was compared with non-pair t-test; The change of renal histology among groups was compared with U-test. Results: Totally 42 rats were involved in the result analysis. (1) The systolic pressure in the exercise training group was similar to that in the control group during experimental process. The systolic pressure in the enalaprol group and exercise training + enalaprol group was lower significantly than that in the control group at 2-8 weeks of experiment (P < 0.01). The excretion volume of urine protein in the exercise training group and enalaprol group was similar; The excretion volume of urine protein in the exercise training + enalaprol group was lower than that in the control group at 4-6 weeks experiment, but the difference was insignificant. (2) The serum total cholesterol in the exercise training group and enalaprol group was lower than that in the control group, but the difference was insignificant, while the levels of serum total cholesterol and triacylglycerol in the exercise training + enalaprol group were lower significantly than those in the control group and exercise training group (P < 0.05-0.01). The differences of blood creatinine, blood uria nitrogen and fasting glucose in every group were insignificant. (3) The index of renal corpuscle lipid deposition in the exercise training group and exercise training + enalaprol group was lower significantly than that in the control group (P < 0.05). The glomerulosclerosis index in the exercise training group was lower than that in the control group, but the difference was insignificant. The glomerulosclerosis index in the exercise training + enalaprol group was lower significantly than that in the control group (P < 0.05). Conclusion: The exercise training has the important effects on improving lipid metabolism in nephritic rats, inhibiting the progress of nephritic damage. The enalaprol can increase the nephritic protective effects on exercise training further.

本文言語English
ページ(範囲)99-101
ページ数3
ジャーナルChinese Journal of Clinical Rehabilitation
9
35
出版ステータスPublished - 2005 9 21

ASJC Scopus subject areas

  • Rehabilitation

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