Effects of dextran sulfates on the acute infection and growth stages of Toxoplasma gondii

Akiko Ishiwa, Kyousuke Kobayashi, Hitoshi Takemae, Tatsuki Sugi, Haiyan Gong, Frances C. Recuenco, Fumi Murakoshi, Atsuko Inomata, Taisuke Horimoto, Kentaro Kato

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Toxoplasma gondii is one of the most prevalent parasites, causing toxoplasmosis in various warm-blooded animals, including humans. Because of the broad range of hosts susceptible to T. gondii, it had been postulated that a universal component of the host cell surface, such as glycosaminoglycans (GAGs), may act as a receptor for T. gondii infection. Carruthers et al. (Infect Immun 68:4005-4011, 2000) showed that soluble GAGs have also been shown to disrupt parasite binding to human fibroblasts. Therefore, we investigated the inhibitory effect of GAGs and their analogue dextran sulfate (DS) on T. gondii infection. For up to 24 h of incubation after inoculation of T. gondii, the inhibitory effect of GAGs on T. gondii infection and growth inside the host cell was weak. In contrast, DS markedly inhibited T. gondii infection. Moreover, low molecular weight DS particularly slowed the growth of T. gondii inside host cells. DS10 (dextran sulfate MW 10 kDa) was the most effective agent in these in vitro experiments and was therefore tested for its inhibitory effects in animal experiments; infection inhibition by DS10 was confirmed under these in vivo conditions. In this report, we showed that DSs, especially DS10, have the potential of a new type of drug for toxoplasmosis.

本文言語English
ページ(範囲)4169-4176
ページ数8
ジャーナルParasitology Research
112
12
DOI
出版ステータスPublished - 2013 12月
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 獣医学(全般)
  • 昆虫科学
  • 感染症

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