Effects of calorie restriction on the age-dependent accumulation of mutations in the small intestine of lacZ-transgenic mice

Dongwei He, Yoshihiko Uehara, Maiko Furuya, Hironobu Ikehata, Jun Ichiro Komura, Kazumi Yamauchi, Shizuko Kakinuma, Yi Shang, Yoshiya Shimada, Akira Ootsuyama, Toshiyuki Norimura, Tetsuya Ono

研究成果: Article査読

1 被引用数 (Scopus)

抄録

To understand the effect of calorie restriction on genome maintenance systems, the age-dependent accumulation of mutations in animals maintained on high and low calorie diets was examined using lacZ-transgenic mice. Mice were fed a diet of 95. kcal/w or 65. kcal/w from 2 to 17 months of age. The mutation frequencies in the lacZ gene in epithelial tissues from the small intestine were examined at 12 and 17 months. Mutation frequencies were found to be lower in mice fed with a low calorie diet than in mice fed with a high calorie diet at the two age points. The molecular nature of the mutations was examined with DNA sequencing. It showed a predominance of transversions from G:C to T:A, and this is a typical type of mutation induced by reactive oxygen species. The fraction of this type of mutation among the different types of mutations detected was not affected by calorie restriction. The percentage of the other types of mutation was not influenced either. These results suggest that calorie restriction reduces the age-dependent accumulation of mutations by stimulating or inducing various types of DNA protection and repair systems rather than protecting cells against any specific type of DNA alteration.

本文言語English
ページ(範囲)117-122
ページ数6
ジャーナルMechanisms of Ageing and Development
132
3
DOI
出版ステータスPublished - 2011 3

ASJC Scopus subject areas

  • 加齢科学
  • 発生生物学

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