Effects of adipokines on expression of adrenomedullin and endothelin-1 in cultured vascular endothelial cells

Kazuhiro Takahashi, Kazuhito Totsune, Masahiko Sone, Kumi Kikuchi, Osamu Murakami

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Obesity is a major risk factor for the development of hypertension. Adipokines may cause hypertension by acting both centrally and directly on the vascular vessels. We wished to clarify whether three adipokines, leptin, resistin and tumor necrosis factor-α, affect expression of adrenomedullin and endothelin-1 in vascular endothelial cells. Human umbilical vein endothelial cells were cultured for 24 h with leptin (1-10 nmol/l), resistin (1-10 nmol/l) or tumor necrosis factor-α (1-10 ng/ml). Expression of adrenomedullin and endothelin-1 was examined by radioimmunoassay and northern blot analysis. Immunoreactive-adrenomedullin in the medium and adrenomedullin mRNA expression levels were decreased by treatment of tumor necrosis factor-α time- and dose-dependently, whereas endothelin-1 secretion was not significantly changed by it. Leptin or resistin had no significant effects on expression of adrenomedullin or endothelin-1 in human umbilical vein endothelial cells. Under hypoxic conditions (1% O2), expression of both adrenomedullin and endothelin-1 was induced in these cells. Immunoreactive-adrenomedullin levels in the medium were decreased by treatment of tumor necrosis factor-α under hypoxia. Leptin or resistin had no significant effects on adrenomedullin or endothelin-1 expression also in hypoxia. These findings have raised the possibility that decreased expression of adrenomedullin by tumor necrosis factor-α may be related to the increased risk of hypertension and other cardiovascular diseases in obese subjects.

本文言語English
ページ(範囲)845-851
ページ数7
ジャーナルPeptides
26
5
DOI
出版ステータスPublished - 2005 5

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 内分泌学
  • 細胞および分子神経科学

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