Effective concentration of ionic liquids for enhanced saccharification of cellulose

Kazuhiko Tanimura, Yoshiko Ooe, Keishi Suga, Hiroshi Umakoshi

研究成果: Article査読

3 被引用数 (Scopus)

抄録

In an aqueous enzymatic saccharification using cellulase, the dissolution of crystalline cellulose is one of the rate-limiting steps. Insoluble cellulose powder was preliminarily heat-treated with ionic liquids (ILs), such as [Bmim][Cl] (1-butyl-3-methylimidazolium chloride) and [Amim][Cl] (1-allyl-3-methylimidazolium chloride), which enable the production of soluble cellulose. On the other hand, the presence of ILs leads to a denaturation of enzymes. Using cellulase from Trichoderma viride, the effects of [Bmim][Cl] and [Amim][Cl] in the enzymatic saccharification were compared. The production of glucose was optimized with 5 wt%-ILs, both for [Bmim][Cl] and for [Amim][Cl]. The significant inhibiting effects of ILs (IL concentration >10 wt%) could be due to the denaturation of cellulase, because the peak shifts of intrinsic tryptophan fluorescence were observed in the presence of 7.5 wt%-ILs. To analyze kinetic parameters, the Langmuir adsorption model and the Michaelis-Menten model were employed. The investigation suggests that [Amim][Cl] can provide soluble cellulose more efficiently, and can promote enzymatic saccharification in the IL concentration below 5 wt%.

本文言語English
論文番号47
ページ(範囲)1-8
ページ数8
ジャーナルChemEngineering
2
4
DOI
出版ステータスPublished - 2018 12
外部発表はい

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • Energy(all)

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