Effect of the structure of bone morphogenetic protein carriers on ectopic bone regeneration

Masaya Yamamoto, Koichi Kato, Yoshito Ikada

研究成果: Article査読

12 被引用数 (Scopus)


To study the effect of the shape of carriers on bone regeneration, two biodegradable polymeric materials, a polyglycolide (PGA) nonwoven fabric and a gelatin hydrogel, were used as carriers of recombinant human bone morphogenetic protein-2 (rhBMP-2). The PGA nonwoven fabric was made from PGA fibers of 20 μm diameter without using any binders while the gelatin hydrogel was prepared by cross-linking of gelatin in aqueous solution with glutaraldehyde to a water content of 95% when swollen with water. Following impregnation of rhBMP-2, the carriers of disk type were implanted into the Wistar rat thigh muscle. The induction of ectopic bone formation from the rhBMP-2-impregnated carriers was evaluated by Softex and histologic observation after staining the explanted tissue with alizarin red S stain to identify calcium deposition. Both of the biodegradable polymeric carriers containing 10 μg of rhBMP-2 induced ectopic bone formation after 2 weeks of implantation but not at the first week after implantation. A remarkable finding was a difference in the macroscopic morphology between the ectopic bones induced by the PGA nonwoven fabric and the gelatin hydrogel. The PGA nonwoven fabric containing rhBMP-2 induced ectopic bone formation inside of the carrier, whereas the gelatin hydrogel formed bone at the periphery of the carrier.

ジャーナルTissue Engineering
出版ステータスPublished - 1996

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 細胞生物学


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