Effect of the bases flanking an abasic site on the recognition of nucleobase by amiloride

Arivazhagan Rajendran, Chunxia Zhao, Burki Rajendar, Viruthachalam Thiagarajan, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Background: We explain here the various non-covalent interactions which are responsible for the different binding modes of a small ligand with DNA. Methods: The combination of experimental and theoretical methods was used. Results: The interaction of amiloride with thymine was found to depend on the bases flanking the AP site and different binding modes were observed for different flanking bases. Molecular modeling, absorption studies and binding constant measurements support for the different binding patterns. The flanking base dependent recognition of AP site phosphates was investigated by 31P NMR experiments. The thermodynamics of the ligand-nucleotide interaction was demonstrated by isothermal titration calorimetry. The emission behavior of amiloride was found to depend on the bases flanking the AP site. Amiloride photophysics in the context of AP-site containing DNA is investigated by time-dependent density functional theory. Conclusions: Flanking bases affect the ground and excited electronic states of amiloride when binding to AP site, which causes flanking base-dependent fluorescence signaling. General significance: The various noncovalent interactions have been well characterized for the determination of nucleic acid structure and dynamics, and protein-DNA interactions. However, these are not clear for the DNA-small molecule interactions and we believe that our studies will bring a new insight into such phenomena.

本文言語English
ページ(範囲)599-610
ページ数12
ジャーナルBiochimica et Biophysica Acta - General Subjects
1800
6
DOI
出版ステータスPublished - 2010 6 1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

フィンガープリント 「Effect of the bases flanking an abasic site on the recognition of nucleobase by amiloride」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル