Effect of Stearylguanidinium-Modified POPC Vesicles on the Melting Behavior of tRNA Molecules

Keishi Suga, Nozomi Watanabe, Hiroshi Umakoshi

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Lipid membranes interact with biomolecules via noncovalent bonding interactions, wherein the physicochemical membrane properties are key factors in the recognition and rearrangement of biomolecule conformation. In this study, vesicles were prepared using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and stearylguanidinium (SG) to improve the affinity between the membrane and tRNA. Membrane fluidity decreased and dehydration of the membrane surface increased with increasing SG levels, suggesting that SG molecules could make the membrane rigid and induce a liquid-ordered (lo)-like phase. The binding constant (Ka) between nucleotide and lipid was evaluated by turbidity analysis; the Ka value for POPC/SG = (86/14) was 2.9 × 104 M-1 but was slightly decreased by vesicles in the lo-like phase. CD spectra of tRNA by the presence of POPC/SG vesicles showed C-G selective base cleavage in tRNA during heat denaturation. POPC/SG = (61/39) and POPC/SG = (40/60) effectively led to C-G base pair cleavage at the melting temperature of tRNA.

本文言語English
ページ(範囲)5662-5669
ページ数8
ジャーナルJournal of Physical Chemistry B
120
25
DOI
出版ステータスPublished - 2016 6 30
外部発表はい

ASJC Scopus subject areas

  • 物理化学および理論化学
  • 表面、皮膜および薄膜
  • 材料化学

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