Effect of a topical steroid on gene expressions for chemokines in mice with contact hypersensitivity

Gaku Mitsui, Takeo Hirano, Yoshimi Niwano, Kazutaka Mitsui, Osamu Ohara, Satoshi Yanagihara, Masatoshi Kato

研究成果: Article査読

14 被引用数 (Scopus)


Effects of a topical corticosteroid drug, diflucortolone valerate, on the mRNA expressions for four CC- and four CXC-chemokines, which have been reported to be associated with recruitment of different kinds of proinflammatory and inflammatory cells, were investigated by RT-PCR in mice with 2,4,6-trinitrochlorobenzene (TNCB)-induced contact hypersensitivity (CHS) response. All of the eight gene expressions were clearly up-regulated in the lesion site of the CHS response up to 24 h post-challenge of TNCB at which ear swelling response reached a peak, so that heavy infiltration of inflammatory cells consisting mainly of mononuclear cells and neutrophils was likely induced by these chemokines. Topical treatment with diflucortolone valerate suppressed completely the infiltrates as well as the ear swelling response. In addition, the up-regulation of gene expressions for these eight chemokines were suppressed by the treatment, indicating that the corticosteroid drug attenuates the expression of chemokine genes essential for orientating nonspecific skin response to hapten-specific CHS response through the recruitment of inflammatory cells from the circulation into the tissue site.

ジャーナルInternational Immunopharmacology
出版ステータスPublished - 2004 1月

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 薬理学


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