EDTA-FISH: A simple and effective approach to reduce non-specific adsorption of probes in fluorescence in situ hybridization (FISH) for environmental samples

Yuki Morono, Kengo Kubota, Daisuke Tsukagoshi, Takeshi Terada

研究成果: Article査読

抄録

Fluorescence in situ hybridization (FISH) is a widely used molecular technique in microbial ecology. However, the non-specific adsorption of fluorescent probes and resulting high intensity of background signals from mineral particles hampers the specific detection of microbial cells in grain-rich environmental samples, such as subseafloor sediments. We herein demonstrated that a new buffer composition containing EDTA efficiently reduced the adsorption of probes without compromising the properties of the FISH-based probing of microbes. The inclusion of a high concentration of EDTA in the buffer in our protocol provides a simple and effective approach for reducing the background in FISH for environmental samples.

本文言語English
論文番号ME20062
ページ(範囲)1-4
ページ数4
ジャーナルMicrobes and environments
35
3
DOI
出版ステータスPublished - 2020

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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