DOSEc, a heme-regulated phosphodiesterase, plays an important role in the regulation of the cyclic AMP level in Escherichia coli

Tokiko Yoshimura-Suzuki, Ikuko Sagami, Nao Yokota, Hirofumi Kurokawa, Toru Shimizu

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Heme-regulated phosphodiesterase from Escherichia coli (DOSEc) catalyzes the hydrolysis of cyclic AMP (cAMP) in vitro and is regulated by the redox state of the bound heme. Changes in the redox state result in alterations in the three-dimensional structure of the enzyme, which is then transmitted to the functional domain to switch catalysis on or off. Because DOSEc was originally cloned from E. coli genomic DNA, it has not been known whether it is actually expressed in wild-type E. coli. In addition, the turnover number of DOSEc using cAMP as a substrate is only 0.15 min-1, which is relatively low for a physiologically relevant enzyme. In the present study, we demonstrated for the first time that the DOSEc gene and protein are expressed in wild-type E. coli, especially under aerobic conditions. We also developed a DOSEc gene knockout strain (Δdos). Interestingly, the knockout of dos caused excess accumulation of intracellular cAMP (26-fold higher than in the wild-type strain) under aerobic conditions, whereas accumulation of cAMP was not observed under anaerobic conditions. We also found differences in cell morphology and growth rate between the mutant cells and the wild-type strain. The changes in the knockout strain were partially complemented by introducing an expression plasmid for dos. Thus, the present study revealed that expression of DOSEc is regulated according to environmental O2 availability at the transcriptional level and that the concentration of cAMP in cells is regulated by DOSEc expression.

本文言語English
ページ(範囲)6678-6682
ページ数5
ジャーナルJournal of bacteriology
187
19
DOI
出版ステータスPublished - 2005 10

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

フィンガープリント

「DOS<sub>Ec</sub>, a heme-regulated phosphodiesterase, plays an important role in the regulation of the cyclic AMP level in Escherichia coli」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル