DNA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure

Toshiyuki Takai, Hitoshi Ohmori

研究成果: Article査読

68 被引用数 (Scopus)

抄録

Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3·10-5 for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively.

本文言語English
ページ(範囲)105-109
ページ数5
ジャーナルBBA - Gene Structure and Expression
1048
1
DOI
出版ステータスPublished - 1990 1月 30
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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