DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

Hirofumi Sato; Yusuke Hashimoto; Mayuko Kikkawa; Takehiko Wada; Yoshihisa Inoue

DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

Hirofumi Sato, Yusuke Hashimoto, Mayuko Kikkawa, Takehiko Wada, Yoshihisa Inoue

研究成果: Article › 査読

抄録

Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

本文言語	English
ページ（範囲）	159-160
ページ数	2
ジャーナル	Nucleic acids research. Supplement (2001)
号	2
出版ステータス	Published - 2002
外部発表	はい

ASJC Scopus subject areas

医学（全般）

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title = "DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.",

abstract = "Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.",

author = "Hirofumi Sato and Yusuke Hashimoto and Mayuko Kikkawa and Takehiko Wada and Yoshihisa Inoue",

year = "2002",

language = "English",

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journal = "Nucleic acids research. Supplement (2001)",

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T1 - DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

AU - Sato, Hirofumi

AU - Hashimoto, Yusuke

AU - Kikkawa, Mayuko

AU - Wada, Takehiko

AU - Inoue, Yoshihisa

PY - 2002

Y1 - 2002

N2 - Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

AB - Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

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JO - Nucleic acids research. Supplement (2001)

JF - Nucleic acids research. Supplement (2001)

IS - 2

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DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

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