DNA methylation and its involvement in carboxylesterase 1A1 (CES1A1) gene expression

T. Hori, M. Hosokawa

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Carboxylesterase 1A1 (CES1A1) efficiently catalyses the hydrolysis of a substrate containing ester, amide, or thioester bonds. It is expressed at a high level in the human liver, but at a low level in the human kidney. In this study, we found the cause of this tissue-specific expression of the CES1A1 gene using 5-aza-2′-deoxycytidine (5-aza-dC) and bisulfite sequencing. Treatment of HEK293 cells, human embryonic kidney cells not expressing the CES1A1 gene, with 5-aza-dC caused dramatic expression of the CES1A1 gene. Bisulfite sequencing revealed that the region around the transcription start site (TSS) of the CES1A1 gene was almost entirely methylated in HEK293 cells, whereas the region was almost entirely unmethylated in HepG2 cells, human hepatoma cells. The hypomethylated DNA molecules for the region were observed in HEK293 cells treated with 5-aza-dC. In the genome obtained from the kidney, the region downstream of the TSS was methylated compared with that obtained from the liver. From these findings, it can be concluded that DNA methylation is involved in CES1A1 gene expression and that the difference between CES1A1 gene expression in the human kidney and that in the human liver may arise from the difference in DNA methylation levels in the region around the TSS.

本文言語English
ページ(範囲)119-128
ページ数10
ジャーナルXenobiotica
40
2
DOI
出版ステータスPublished - 2010 2月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 毒物学
  • 薬理学
  • 健康、毒物学および変異誘発

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