DNA Methylation and Expression of the Rat Pepsinogen Gene in Embryonic, Adult, and Neoplastic Tissues

Masao Ichinose, Kazumasa Miki, Chie Furihata, Masae Tatematsu, Yoshikazu Ichihara, Takeshi Ishihara, Isao Katsura, Kazuhiro Sogawa, Yoshiaki Fujii-Kuriyama, Masao Tanji Hiroshi Oka, Taijiro Matsushima, Kenii Takahashi

    研究成果: Article査読

    34 被引用数 (Scopus)

    抄録

    The relationship between methylation and expression of rat pepsinogen 1 (Pgl) genes was investigated in various tissues. On Northern blotting with a Pgl complementary DNA probe, Pgl mRNA was detected only in the glandular stomach of normal rats. Methylation analysis with Msp1/HpaII and Hha1 revealed tissue specific methylation patterns of Pgl genes with less methylated in the stomach than in other normal tissues not expressing the genes. During stomach development, there was a progressive increase in the Pgl mRNA level that almost coincided with change in the mucosal pepsinogen level and progressive demethylation after the onset of transcription. Thus, there was an inverse correlation between methylation and expression of Pgl genes, suggesting a role of DNA methylation in Pgl gene regulation during normal differentiation, although not its primary role in gene activation. There was no detectable Pgl mRNA in either primary or transplanted stomach cancers induced by W-methyl-AT -nitro-7V-nitrosoguanidine. The methylation patterns of Pgl genes were different from those of normal tissues that expressed the gene and of those that did not and no simple correlation was observed between methylation and expression of Pgl genes. This result is consistent with a previous finding that DNA methylation is deranged in tumor cells.

    本文言語English
    ページ(範囲)1603-1609
    ページ数7
    ジャーナルCancer Research
    48
    6
    出版ステータスPublished - 1988

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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