DNA-binding properties of Mblk-1, a putative transcription factor from the honeybee

Jung Min Park, Takekazu Kunieda, Hideaki Takeuchi, Takeo Kubo

研究成果: Article査読

20 被引用数 (Scopus)

抄録

We previously identified a gene, Mblk-1, that encodes a putative transcription factor with two DNA-binding motifs expressed preferentially in the honeybee brain [H. Takeuchi et al., Insect Mol. Biol. 10, 487-494 (2001)]. In the present study, we identified its preferred binding sequence as 5′-CCCTATCGATCG-ATCTCTACCT-3′ and characterized its DNA-binding properties using truncated Mblk-1 mutants. An electrophoretic mobility shift assay revealed that the full-length Mblk-1 binds to the sequence with high affinity, whereas each truncated DNA-binding motif of Mblk-1 binds with much lower affinities. An in vitro pull-down assay indicated that each DNA-binding motif affords homodimeric bindings, suggesting that Mblk-1 functions as a dimer.

本文言語English
ページ(範囲)23-28
ページ数6
ジャーナルBiochemical and biophysical research communications
291
1
DOI
出版ステータスPublished - 2002 2月 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「DNA-binding properties of Mblk-1, a putative transcription factor from the honeybee」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル