Distribution of Retinoids Applied Exogenously to Chick Limb Buds: an Autoradiographic Analysis: Retinoic Acid/Limb Bud/Pattern Formation/Autoradiograph

Koji Tamura, Yuichi Hashimoto, Koichi Shudo, Hiroyuki Ide

研究成果: Article査読

4 被引用数 (Scopus)

抄録

An autoradiographic analysis was undertaken to examine the localization of retinoids applied exogenously to chick limb buds. Ion‐exchange beads (AGI‐X2) containing a tritium‐labeled synthetic retinoid, Am80, were implanted to various regions of chick wing buds. This synthetic retinoid is known to induce a duplicated limb pattern as retinoic acid (RA) does. One to 24 hours after the application, wing buds were fixed, sectioned, and prepared for autoradiography. Heavy labeling was observed in the peripheral region of the wing mesoderm, but no gradient along the antero‐posterior axis was found. These results suggest that the peripheral region of the limb bud may be important for the morphogenetic function of RA. Tissue‐bound retinoids may not form an antero‐posterior concentration gradient when retinoids are added to the anterior margin of the chick limb bud.

本文言語English
ページ(範囲)593-599
ページ数7
ジャーナルDevelopment, Growth & Differentiation
35
5
DOI
出版ステータスPublished - 1993 10

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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