Disruption of the mouse protein Ser/Thr phosphatase 2Cβ gene leads to early pre-implantation lethality

Masato Sasaki, Motoko Ohnishi, Fumi Tashiro, Hitoshi Niwa, Akira Suzuki, Jun ichi Miyazaki, Takayasu Kobayashi, Shinri Tamura

研究成果: Article査読

22 被引用数 (Scopus)

抄録

Protein phosphatase 2Cβ (PP2Cβ) is a member of a family of protein Ser/Thr phosphatases (PP2C) that is composed of at least twelve different gene products. Recent studies have revealed that PP2Cβ mRNA accumulates in mature sperm, unfertilized metaphase II-arrested oocytes and zygotes, but that the mRNA level then decreases sharply between the early two-cell and eight-cell stages, remaining at low levels during the 16-cell to blastocyst stages of mice. These observations raised the possibility that PP2Cβ plays a crucial role during gametogenesis, fertilization, and/or early stages of embryonic development. In this study, we employed a gene knockout technique in mice to test this possibility. We found that PP2CβΔ/wt mice generate normal mature gametes. However, PP2CβΔ/Δ embryos die between the two-cell and eight-cell stages. To our interest, PP2CβΔ/Δ ES cells which had been generated by transfecting PP2Cβ3lox/3lox ES cells with Cre-expressing plasmid were viable. In addition, knockdown of PP2Cβ using siRNA did not affect the proliferation of wild-type ES cells. These observations suggest that relatively high PP2Cβ expression is specifically required during the early stages of pre-implantation development. The possible mechanisms for the early pre-implantation lethality of PP2CβΔ/Δ mice are discussed.

本文言語English
ページ(範囲)489-499
ページ数11
ジャーナルMechanisms of Development
124
6
DOI
出版ステータスPublished - 2007 7

ASJC Scopus subject areas

  • 胎生学
  • 発生生物学

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