Dispersion and degradation of environmental DNA from caged fish in a marine environment

Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda

研究成果: Article

12 引用 (Scopus)

抜粋

Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.

元の言語English
ページ(範囲)327-337
ページ数11
ジャーナルFisheries Science
85
発行部数2
DOI
出版物ステータスPublished - 2019 3 15

ASJC Scopus subject areas

  • Aquatic Science

フィンガープリント Dispersion and degradation of environmental DNA from caged fish in a marine environment' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Murakami, H., Yoon, S., Kasai, A., Minamoto, T., Yamamoto, S., Sakata, M. K., Horiuchi, T., Sawada, H., Kondoh, M., Yamashita, Y., & Masuda, R. (2019). Dispersion and degradation of environmental DNA from caged fish in a marine environment. Fisheries Science, 85(2), 327-337. https://doi.org/10.1007/s12562-018-1282-6