Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis

Shin ichiro M. Nomura, Satoshi Kondoh, Wakiko Asayama, Akikazu Asada, Shigemichi Nishikawa, Kazunari Akiyoshi

研究成果: Article査読

62 被引用数 (Scopus)

抄録

We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b5 (b5) and its fusion proteins were synthesized and directly localized in the liposomes. After the translation reaction, the proteo-liposomes were isolated by simplified discontinuous density-gradient centrifugation. Apo cytochrome b5 conjugated dihydrofolate reductase (DHFR) was synthesized in the same procedure and the protein was directly displayed on the liposome surface. b5 acts as a "hydrophobic tag" for recruitment to the liposome surface.

本文言語English
ページ(範囲)190-195
ページ数6
ジャーナルJournal of Biotechnology
133
2
DOI
出版ステータスPublished - 2008 1 20
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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