Direct observation of specific interaction between enzyme-substrate complexes using colloidal probe atomic force microscopy

Takehiro Suzuki, Yuan Wei Zhang, Tanetoshi Koyama, Darryl Y. Sasaki, Kazue Kurihara

研究成果: Article査読

4 被引用数 (Scopus)

抄録

The interactions between the enzyme-substrate complex of heptaprenyl diphosphate synthase were directly investigated using colloidal probe atomic force microscopy. This enzyme is composed of two dissociable subunits which exhibit a catalytic activity only when they are associated together in the presence of Mg2+ and farnesyl diphosphate (FPP). We observed the adhesive force between the subunits only in the presence of both Mg2+ (0.1 mM) and FPP (15 μM). This is the first direct demonstration of the specific interaction involved in the enzyme reaction.

本文言語English
ページ(範囲)536-537
ページ数2
ジャーナルChemistry Letters
33
5
DOI
出版ステータスPublished - 2004 5 5

ASJC Scopus subject areas

  • Chemistry(all)

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