Direct Observation of Amyloid β Behavior at Phospholipid Membrane Constructed on Gold Nanoparticles

Keishi Suga, Ying Chen Lai, Miftah Faried, Hiroshi Umakoshi

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Amyloid β (Aβ) is a potential biomarker of Alzheimer's disease (AD), and its fibrillation behavior is of interest and value. In this study, the Aβ behaviors on phospholipid membranes were observed by Membrane Surface-Enhanced Raman Spectroscopy (MSERS) method. Phospholipid (PL) membranes, consisting of DMPC and DMPS with a molar ratio of 9:1, were fabricated on gold nanoparticles with diameter of 100 nm (Au@PL). Enhancement of the Raman intensity of Au@PL was increased by Aβ, with enhancement factor about 40. The H-bonding network was disturbed in presence of NaCl which covered Au@PL and made Au@PL away from one another. When Aβ was applied with Au@PL, the H-bonding network was disturbed just after mixing. As the reaction reaches to equilibrium, Aβ attracted neighbouring Au@PL and induced aggregation of Au@PL which blocked the aggregation prone site of Aβ to inhibit further fibrillation. Based on our method, the Aβ behaviors at lipid membrane surface can be directly observed via enhanced Raman signals.

本文言語English
論文番号2571808
ジャーナルInternational Journal of Analytical Chemistry
2018
DOI
出版ステータスPublished - 2018
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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