Direct measurement of phagosomal reactive oxygen by luminol-binding microspheres

T. Uchida, T. Kanno, S. Hosaka

研究成果: Article査読

20 被引用数 (Scopus)

抄録

A new method, utilizing microsphere-bound luminol, which makes possible the direct measurement of highly reactive oxygen within phogosomes, was studied. When Freund's complete adjuvant-elicited mouse peritoneal macrophages and luminol-binding microspheres were mixed, the microspheres were engulfed in macrophages and enclosed in phagosomes, where chemiluminescence (CL) was generated, showing the generation of highly reactive oxygen. The reactive oxygen could be quantitatively assayed by measuring the intensity of CL. The addition of cytochalasin B inhibited the CL. CL production by the thioglycollate-elicited macrophages was found to be only a ninth of that by Freund's complete adjuvant-elicited macrophages, though the phagocytic activities were almost equivalent in both cases.

本文言語English
ページ(範囲)55-61
ページ数7
ジャーナルJournal of Immunological Methods
77
1
DOI
出版ステータスPublished - 1985 2 28

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

フィンガープリント

「Direct measurement of phagosomal reactive oxygen by luminol-binding microspheres」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル