抄録
A new method of directly measuring esterase activity within phagolysosomes has been developed. Decanoyl fluorescein- binding microspheres were prepared and phagocytosed by human peripheral neutrophils. Within phagolysoscmes lysosomal esterase hydrolyzed decanoyl fluorescein on the microspheres, causing the conversion of decanoyl fluorescein- binding microspheres (non-fluorescent) into fluorescein- binding microspheres (fluorescent). The activity of phagolysosomal esterase in intact neutrophils was assayed by the measurement of the fluorescence intensity without rupturing cells. By use of a flow cytometer, esterase activity within phagolysoscmes in single cells was measured.
本文言語 | English |
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ページ(範囲) | 584-589 |
ページ数 | 6 |
ジャーナル | Biochemical and biophysical research communications |
巻 | 127 |
号 | 2 |
DOI | |
出版ステータス | Published - 1985 3月 15 |
ASJC Scopus subject areas
- 生物理学
- 生化学
- 分子生物学
- 細胞生物学