Direct measurement of phagolysosomal esterase activity

Takafumi Uchida, Shuntaro Hosaka, Kumiko Miura

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A new method of directly measuring esterase activity within phagolysosomes has been developed. Decanoyl fluorescein- binding microspheres were prepared and phagocytosed by human peripheral neutrophils. Within phagolysoscmes lysosomal esterase hydrolyzed decanoyl fluorescein on the microspheres, causing the conversion of decanoyl fluorescein- binding microspheres (non-fluorescent) into fluorescein- binding microspheres (fluorescent). The activity of phagolysosomal esterase in intact neutrophils was assayed by the measurement of the fluorescence intensity without rupturing cells. By use of a flow cytometer, esterase activity within phagolysoscmes in single cells was measured.

本文言語English
ページ(範囲)584-589
ページ数6
ジャーナルBiochemical and biophysical research communications
127
2
DOI
出版ステータスPublished - 1985 3 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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