Direct measurement of phagolysosomal esterase activity

Takafumi Uchida, Shuntaro Hosaka, Kumiko Miura

    研究成果: Article査読

    6 被引用数 (Scopus)

    抄録

    A new method of directly measuring esterase activity within phagolysosomes has been developed. Decanoyl fluorescein- binding microspheres were prepared and phagocytosed by human peripheral neutrophils. Within phagolysoscmes lysosomal esterase hydrolyzed decanoyl fluorescein on the microspheres, causing the conversion of decanoyl fluorescein- binding microspheres (non-fluorescent) into fluorescein- binding microspheres (fluorescent). The activity of phagolysosomal esterase in intact neutrophils was assayed by the measurement of the fluorescence intensity without rupturing cells. By use of a flow cytometer, esterase activity within phagolysoscmes in single cells was measured.

    本文言語English
    ページ(範囲)584-589
    ページ数6
    ジャーナルBiochemical and biophysical research communications
    127
    2
    DOI
    出版ステータスPublished - 1985 3月 15

    ASJC Scopus subject areas

    • 生物理学
    • 生化学
    • 分子生物学
    • 細胞生物学

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