Direct electroporation of adherent cells by hydrogel-based microelectrodes

M. Nishizawa, K. Nagamine

研究成果: Conference contribution

抜粋

We have developed a technique to fabricate Au microelectrodes on hydrogel substrates such as polyacrylamide, agarose, collagen etc. Electropolymerized poly (3,4-ethylenedioxythiophene) (PEDOT) serves as the anchor for bonding the Au electrodes to the hydrogels. The prepared soft hydrogel-based electrode can be directly laminated to adherent cells for in-situ electroporation. Owing to the molecular permeability of the hydrogels, continuous post-cultivation with the laminated electrode substrate was also possible. The electroporation- assisted uptakes of dye molecules and genes were demonstrated for normal human dermal fibroblast cells and human iPS cells.

元の言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版者Chemical and Biological Microsystems Society
ページ1734-1736
ページ数3
ISBN(印刷物)9781632666246
出版物ステータスPublished - 2013 1 1
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Germany
Freiburg
期間13/10/2713/10/31

ASJC Scopus subject areas

  • Bioengineering

フィンガープリント Direct electroporation of adherent cells by hydrogel-based microelectrodes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Nishizawa, M., & Nagamine, K. (2013). Direct electroporation of adherent cells by hydrogel-based microelectrodes. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1734-1736). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; 巻数 3). Chemical and Biological Microsystems Society.