Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism

Takuichi Sato, Junko Matsuyama, Nobuhiro Takahashi, Michiko Sato, Jolene Johnson, Charles Schachtele, Etsuro Hoshino

研究成果: Article査読

17 被引用数 (Scopus)

抄録

16S rDNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to generate restriction profiles of the reference strains, including the American Type Culture Collection type strains, of oral Actinomyces spp., i.e., A. israelii, A. gerencseriae, A. naeslundii genospecies 1 and 2, A. odontolyticus, A. meyeri and A. georgiae, and 23 Actinomyces strains isolated from human dental plaque. The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. The PCR products were purified and characterized by single digestion with four restriction endonucleases, i.e., MnlI, HaeIII, CfoI, or HpaII. Among them, Mn/I was found to discriminate the respective reference strains. The clinical isolates were assigned to one of the species, i.e., A. gerencseriae, A. naeslundii genospecies 1 and 2 and A. odontolyticus, on the basis of their restriction profiles by single digestion with MnlI. Thus, 16S rDNA PCR-RFLP, using MnlI, is a rapid and reliable method for the differentiation of oral Actinomyces spp.

本文言語English
ページ(範囲)247-252
ページ数6
ジャーナルArchives of Oral Biology
43
3
DOI
出版ステータスPublished - 1998 3

ASJC Scopus subject areas

  • 耳鼻咽喉科学
  • 歯科学(全般)
  • 細胞生物学

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