Differentiation and monitoring of cells using a biochip for regenerative medicine (differentiation of bone marrow stromal cells and myoblasts)

Fumihito Arai, Osamu Suzuki, Tomoyuki Uchida, Akihiko Ichikawa, Toshio Fukuda, Takenobu Katagiri, Ryutaro Kamijo, Masanori Nakamura, Mamoru Numata, Naruaki Watanabe

研究成果: Review article査読

5 被引用数 (Scopus)

抄録

A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST 2 cells) and myoblasts (C 2 C 12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.

本文言語English
ページ(範囲)3239-3245
ページ数7
ジャーナルNihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
71
11
DOI
出版ステータスPublished - 2005 11
外部発表はい

ASJC Scopus subject areas

  • 材料力学
  • 機械工学
  • 産業および生産工学

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