Differential potencies of effector genes in adult Drosophila

Andreas S. Thum, Stephan Knapek, Jens Rister, Eva Dierichs-Schmitt, Martin Heisenberg, Hiromu Tanimoto

研究成果: Article査読

53 被引用数 (Scopus)

抄録

The GAL4/UAS gene expression system in Drosophila has been crucial in revealing the behavioral significance of neural circuits. Transgene products that block neurotransmitter release and induce cell death have been proved to inhibit neural function powerfully. Here we compare the action of the five effector genes shibirets1, Tetanus toxin light chain (TNT), reaper, Diphtheria toxin A-chain (DTA), and inwardly rectifying potassium channel (Kir2.1) and show differences in their efficiency depending on the target cells and the timing of induction. Specifically, effectors blocking neuronal transmission or excitability led to adult-induced paralysis more efficiently than those causing cell ablation. We contrasted these differential potencies in adult to their actions during development. Furthermore, we induced TNT expression in the adult mushroom bodies. In contrast to the successful impairment in short-term olfactory memory by shibirets1, adult TNT expression in the same set of cells did not lead to any obvious impairment. Altogether, the efficiency of effector genes depends on properties of the targeted neurons. Thus, we conclude that the selection of the appropriate effector gene is critical for evaluating the function of neural circuits.

本文言語English
ページ(範囲)194-203
ページ数10
ジャーナルJournal of Comparative Neurology
498
2
DOI
出版ステータスPublished - 2006 9月 10
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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