TY - JOUR
T1 - Differential level in co-down-modulation of CD4 and CXCR4 primed by HIV- 1 gp120 in response to phorbol ester, PMA, among HIV-1 isolates
AU - Tahara-Hanaoka, Satoko
AU - Ushijima, Yuki
AU - Tarui, Hiroshi
AU - Wada, Masayuki
AU - Hara, Toshio
AU - Imanishi, Shigeo
AU - Yamaguchi, Tomoyuki
AU - Hattori, Toshio
AU - Nakauchi, Hiromitsu
AU - Koito, Atsushi
PY - 2000
Y1 - 2000
N2 - HIV-1 enters cells through interacting with cell surface molecules such as CD4 and chemokine receptors. We generated recombinant soluble gp120s derived from T-cell line-tropic (T-tropic) and macrophage-tropic (M-tropic) HIV-1 strains using a baculovirus expression system and investigated the association of CD4-gp120 complex with the chemokine receptor and/or other surface molecule(s). For monitoring the co-down-modulations of the CD4-gp120 complex, a cytoplasmic domain deletion mutant (tailless CD4), which is not capable of undergoing down-modulation by itself in response to phorbol ester PMA, was used. Our studies revealed both cell-type and HIV-1 strain-specific differences. We found that T-tropic gp120s were capable of priming co-down- modulation with tailless CD4 by interacting with CXCR4, whereas M-tropic SF162 gp120 could not after PMA treatment even in the presence of CCR5. Among the T-tropic HIV-1 envelopes, IIIB gp120 was the most potent. Furthermore, the ability of gp120 to prime the PMA induced co-down-modulation of tailless CD4 appeared to be dependent on the concentration of the principal coreceptor CXCR4. Nevertheless, the observation that IIIB gp120 strongly primed tailless CD4 co-down-modulation on human osteosarcoma HOS cells that express undetectable levels of surface CXCR4 raised the possibility that membrane component(s) other than those recently identified can be involved in down- modulation of the CD4/gp120 complexes.
AB - HIV-1 enters cells through interacting with cell surface molecules such as CD4 and chemokine receptors. We generated recombinant soluble gp120s derived from T-cell line-tropic (T-tropic) and macrophage-tropic (M-tropic) HIV-1 strains using a baculovirus expression system and investigated the association of CD4-gp120 complex with the chemokine receptor and/or other surface molecule(s). For monitoring the co-down-modulations of the CD4-gp120 complex, a cytoplasmic domain deletion mutant (tailless CD4), which is not capable of undergoing down-modulation by itself in response to phorbol ester PMA, was used. Our studies revealed both cell-type and HIV-1 strain-specific differences. We found that T-tropic gp120s were capable of priming co-down- modulation with tailless CD4 by interacting with CXCR4, whereas M-tropic SF162 gp120 could not after PMA treatment even in the presence of CCR5. Among the T-tropic HIV-1 envelopes, IIIB gp120 was the most potent. Furthermore, the ability of gp120 to prime the PMA induced co-down-modulation of tailless CD4 appeared to be dependent on the concentration of the principal coreceptor CXCR4. Nevertheless, the observation that IIIB gp120 strongly primed tailless CD4 co-down-modulation on human osteosarcoma HOS cells that express undetectable levels of surface CXCR4 raised the possibility that membrane component(s) other than those recently identified can be involved in down- modulation of the CD4/gp120 complexes.
KW - Coreceptor
KW - Down-modulation
KW - HIV-1
KW - Phorbol ester
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U2 - 10.1111/j.1348-0421.2000.tb02524.x
DO - 10.1111/j.1348-0421.2000.tb02524.x
M3 - Article
C2 - 10941932
AN - SCOPUS:0033920498
VL - 44
SP - 489
EP - 498
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 6
ER -