Differential cytokine gene expression in CD4+ and CD8+ T cell subsets of calves

Sachi Tanaka, Hisashi Aso, Kohtaro Miyazawa, Yasuhiro Nagai, Kouichi Watanabe, Shyuichi Ohwada, Jin Kobayashi, Takahiro Yamaguchi

研究成果: Article

12 引用 (Scopus)

抜粋

The distinct patterns of cytokine expression in CD4+ and CD8+ T cells are well understood in mice and humans. However, little information is available about cytokine expression in bovine CD4+ and CD8+ T cells. In this study, mRNA expression of 19 different cytokines was analyzed in CD4+ and CD8+ T cells of calves with or without Concanavalin A (Con A) stimulation. CD4+ and CD8+ T cell populations were enriched to 98% purity by positive selection using magnetic cell sorting (MACS). CD4+ T cells spontaneously expressed the mRNAs of interleukin-1α (IL-1α), IL-1β, IL-2, IL-6, IL-7, IL-8, IL-10, IL-18, IFN-γ, TNF-α, TNF-β and TGF-β, and augmented the mRNA expression of IL-10, IFN-γ and TNF-β after Con A stimulation. The mRNAs of IL-3, IL-4, IL-5, IL-13 and GM-CSF were newly expressed in Con A-stimulated CD4+ T cells. CD8+ T cells displayed spontaneous mRNA expression of IL-6, IL-18, TNF-α, TNF-β and TGF-β, and newly expressed the mRNA of IL-2, IL-7, interferon-gamma (IFN-γ) and GM-CSF after Con A stimulation. It was found that CD4+ T cells expressed the mRNA of 17 cytokines except for IL-12 and IL-15, while CD8+ T cells expressed only the mRNA of 9 cytokines after Con A stimulation. The profile of cytokine mRNA expression was substantially different in the CD4+ and CD8+ T cells of calves, indicating that CD4+ T cells can be distinguished from CD8+ T cells by the cytokine gene expression of IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-8, IL-10 and IL-13. Differential cytokine expression between CD4+ and CD8+ T cells serve to interpret an individual function of T cell subsets in the immune system of calves.

元の言語English
ページ(範囲)84-91
ページ数8
ジャーナルVeterinary Immunology and Immunopathology
118
発行部数1-2
DOI
出版物ステータスPublished - 2007 7 15

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

フィンガープリント Differential cytokine gene expression in CD4<sup>+</sup> and CD8<sup>+</sup> T cell subsets of calves' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Tanaka, S., Aso, H., Miyazawa, K., Nagai, Y., Watanabe, K., Ohwada, S., Kobayashi, J., & Yamaguchi, T. (2007). Differential cytokine gene expression in CD4+ and CD8+ T cell subsets of calves. Veterinary Immunology and Immunopathology, 118(1-2), 84-91. https://doi.org/10.1016/j.vetimm.2007.04.013