Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens

Naoki Matsumoto, Mina Okochi, Miyoko Matsushima, Akiko Ogawa, Tomokazu Takase, Yasuko Yoshida, Mitsuo Kawase, Ken ichi Isobe, Tsutomu Kawabe, Hiroyuki Honda

研究成果: Article

12 引用 (Scopus)

抜粋

Peptide arrays have become versatile tools for high throughput screening assays in biomedical and pharmaceutical research. In this study, we constructed a peptide array that contained linear peptide fragments reported as IgE-binding epitopes for cow's milk allergy (CMA). Various peptides with different solubility in aqueous solutions were dissolved in the buffer solutions containing sodium dodecyl sulfate, and we achieved a consistent spotting of peptide solutions using a piezoelectric ceramic micropump. The IgE-binding patterns were successfully detected by observing the binding of Alexa 647-labeled anti-human IgE using sera from CMA patients. Our technique in this study will provide a potent capability for the development of a peptide array for mapping IgE-epitopes in milk proteins, and it will help researchers better understand the IgE-epitopes associated with the clinical outcome of CMA.

元の言語English
ページ(範囲)324-330
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
107
発行部数3
DOI
出版物ステータスPublished - 2009 3 1
外部発表Yes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • これを引用

    Matsumoto, N., Okochi, M., Matsushima, M., Ogawa, A., Takase, T., Yoshida, Y., Kawase, M., Isobe, K. I., Kawabe, T., & Honda, H. (2009). Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens. Journal of Bioscience and Bioengineering, 107(3), 324-330. https://doi.org/10.1016/j.jbiosc.2008.10.014