Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens

Naoki Matsumoto, Mina Okochi, Miyoko Matsushima, Akiko Ogawa, Tomokazu Takase, Yasuko Yoshida, Mitsuo Kawase, Ken ichi Isobe, Tsutomu Kawabe, Hiroyuki Honda

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Peptide arrays have become versatile tools for high throughput screening assays in biomedical and pharmaceutical research. In this study, we constructed a peptide array that contained linear peptide fragments reported as IgE-binding epitopes for cow's milk allergy (CMA). Various peptides with different solubility in aqueous solutions were dissolved in the buffer solutions containing sodium dodecyl sulfate, and we achieved a consistent spotting of peptide solutions using a piezoelectric ceramic micropump. The IgE-binding patterns were successfully detected by observing the binding of Alexa 647-labeled anti-human IgE using sera from CMA patients. Our technique in this study will provide a potent capability for the development of a peptide array for mapping IgE-epitopes in milk proteins, and it will help researchers better understand the IgE-epitopes associated with the clinical outcome of CMA.

本文言語English
ページ(範囲)324-330
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
107
3
DOI
出版ステータスPublished - 2009 3月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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