@article{262c181e655f43ea9633cdabf2511165,
title = "Development of localized gene delivery using a dual-intensity ultrasound system in the bladder",
abstract = "A dual-intensity ultrasound system (DIUS) using nanobubbles offers opportunities for localized gene delivery. This system consists of low-/high-ultrasound intensities. The bladder is a balloon-shaped closed organ in which the behavior of nanobubbles can be controlled spatially and temporally by ultrasound exposure. We hypothesized that when a DIUS with nanobubbles was used, low-intensity ultrasound would direct nanobubbles to targeted cells in the bladder, whereas high-intensity ultrasound intensity would collapse nanobubbles and increase cell membrane permeability, facilitating entry of exogenous molecules into proximate cells. A high-frequency ultrasound imaging system characterized movement and fragmentation of nanobubbles in the bladder. Confocal microscopy revealed that fluorescent molecules were delivered in the localized bladder wall, whereas histochemical examination indicated that the molecular transfer efficiency depended on the acoustic energy. A bioluminescence imaging system showed luciferase plasmid DNA was actually transfected in the bladder wall and subsequent transfection depended on acoustic energy. These findings indicate that delivery of exogenous molecules in the bladder using this approach results in high localization of molecular delivery, facilitating gene therapy for bladder cancer.",
keywords = "Acoustic liposome, Bladder cancer, Drug delivery system, Localized gene delivery, Nanobubble, Ultrasound",
author = "Sachiko Horie and Yukiko Watanabe and Rui Chen and Shiro Mori and Yasuhiro Matsumura and Tetsuya Kodama",
note = "Funding Information: We are sincerely grateful to Dr. Masao Ono (Department of Pathology, Tohoku University Graduate School of Medicine) for providing technical advice on pathological analysis as well as constructive feedback and warm encouragement. We would also like to thank Dr. Li (Department of Radiology, Tohoku University Graduate School of Medicine). S. Horie received a Grant-in-Aid for JSPS Fellows ( 21-7271 ) and Grant-in-Aid for Young Scientists (Start-up) ( 19800002 ). Y. Watanabe received a Grant-in-Aid for JSPS Fellows ( 21-7073 ). R. Chen received a Grant-in-Aid for Scientific Research for JSPS Postdoctoral fellowship For Foreign Researchers ( P09127 ). S. Mori received a Grant-in-Aid for Scientific Research (B) ( 19390507 ). Y. Matsumura received a Grant for Research on Advanced Medical Technology, Ministry of Health, Labour and Welfare of Japan ( H17-nano-006 ). T. Kodama received a Grant-in-Aid for Scientific Research (B) ( 20300173 ); Grant-in-Aid for Exploratory Research ( 21650124 ); Grant-in-Aid for Scientific Research on Priority Area, MEXT ( 20015005 ); and a Grant for Research on Advanced Medical Technology, Ministry of Health, Labour and Welfare of Japan ( H19-nano-010 ).",
year = "2010",
month = nov,
doi = "10.1016/j.ultrasmedbio.2010.07.015",
language = "English",
volume = "36",
pages = "1867--1875",
journal = "Ultrasound in Medicine and Biology",
issn = "0301-5629",
publisher = "Elsevier USA",
number = "11",
}