Development of analytical methods to evaluate SFH

K. Nakai, H. Abe, N. Matsuda, M. Kobayashi, H. Ikeda, S. Sekiguchi, E. Tsuchida

研究成果: Article査読

4 被引用数 (Scopus)

抄録

For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40nm and the ultrafiltration method with a 100kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.

本文言語English
ページ(範囲)447-451
ページ数5
ジャーナルBiomaterials, Artificial Cells and Immobilization Biotechnology
20
4 --Feb
DOI
出版ステータスPublished - 1992 1
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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