Development of a novel enhanced biosensor system for real-time monitoring of fish stress using a self-assembled monolayer

Haiyun Wu, Yuzu Fujii, Toshiki Nakano, Takafumi Arimoto, Masataka Murata, Haruto Matsumoto, Yasutoshi Yoshiura, Hitoshi Ohnuki, Hideaki Endo

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Wireless biosensor systems were developed in our lab for monitoring blood glucose concentrations in fish as an indicator of fish stress. However, uniform immobilization of the enzyme on the surface of the electrode is difficult, so the sensor response is typically reduced at a range of high glucose concentrations during the stress monitoring. In this study, we attempted to enhance sensor response by using a self-assembled monolayer-immobilized enzyme. Glucose oxidase was immobilized on a working electrode modified with a self-assembled monolayer. The proposed biosensor showed a good correlation between the output current and the glucose concentration range of 10-3500 mg dL -1 under an optimized working condition. The dynamic measurement range of this newly developed sensor is significantly improved, especially over a high concentration range, which helps the sensor to achieve better performance in dramatic changes in the stress response of fish. In addition, we used biological samples from test fish and obtained a good correlation coefficient between the sensor output current and the glucose concentration using a conventional method. The proposed wireless biosensor system was also applied to monitor fish stress responses in real time through different stressors and to obtain some precise data that reflect real fish stress responses.

本文言語English
論文番号1518
ジャーナルSensors (Switzerland)
19
7
DOI
出版ステータスPublished - 2019 4 1

ASJC Scopus subject areas

  • 分析化学
  • 情報システム
  • 原子分子物理学および光学
  • 生化学
  • 器械工学
  • 電子工学および電気工学

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